Journal: Frontiers in Microbiology

Article Title: Woodfordia fruticosa fermented with lactic acid bacteria impact on foodborne pathogens adhesion and cytokine production in HT-29 cells

doi: 10.3389/fmicb.2024.1346909

Figure Lengend Snippet: Antibacterial activity of WF extracts at various ethanol concentrations (A) . Inhibitory effects of LAB-fermented WF on foodborne pathogens [ Escherichia coli O157:H7 (ATCC 43895) (B) , Listeria monocytogenes (ATCC 7644) (C) , Salmonella typhimurium (ATCC 35987) (D) , and Vibrio parahaemolyticus (ATCC 35118) (E) ]. Values are expressed as mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001. ET, 50% ethanol solution; WF, Woodfordia fruticosa ; WFLR, Woodfordia fruticosa flower fermented with Lacticaseibacillus rhamnosus ATCC9595; WFLA, Woodfordia fruticosa flower fermented with Lacticaseibacillus acidophilus ATCC 4356; WFLC, Woodfordia fruticosa flower fermented with Lacticaseibacillus casei ATCC 7469; WFLB, Woodfordia fruticosa flower fermented with Lactobacillus buchneri KCTC5064.

Article Snippet: The study investigated how LAB fermented WF ( ) affects the growth inhibition of various pathogens, including E. coli O157:H7 (ATCC 43895), L. monocytogenes (ATCC 7644), S. typhimurium (ATCC 35987), and V. parahaemolyticus (ATCC 35118), using the broth dilution technique.

Techniques: Activity Assay

Journal: Frontiers in Microbiology

Article Title: Woodfordia fruticosa fermented with lactic acid bacteria impact on foodborne pathogens adhesion and cytokine production in HT-29 cells

doi: 10.3389/fmicb.2024.1346909

Figure Lengend Snippet: Impact of LAB-fermented WF on HT-29 cell viability with and without foodborne pathogens at 24 and 48 h. (A) HT-29 cell viability in the absence of foodborne pathogens. CT refers to only HT-29 cells. (B) HT-29 cell viability after exposure to Escherichia coli O157:H7 (ATCC 43895) (CT) alone and in combination with LAB-fermented WF. (C) HT-29 cell viability after exposure to Listeria monocytogenes (ATCC 7644) (CT) alone and in combination with LAB-fermented WF. (D) HT-29 cell viability after exposure to Salmonella typhimurium (ATCC 35987) (CT) alone and in combination with LAB-fermented WF. (E) HT-29 cell viability after exposure to Vibrio parahaemolyticus (ATCC 35118) (CT) alone and in combination with LAB-fermented WF. Values are expressed as mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001. WFLR, Woodfordia fruticosa flower fermented with Lacticaseibacillus rhamnosus ATCC9595; WFLA, Woodfordia fruticosa flower fermented with Lactobacillus acidophilus ATCC 4356; WFLC, Woodfordia fruticosa flower fermented with Lactobacillus casei ATCC 7469; WFLB, Woodfordia fruticosa flower fermented with Lactobacillus buchneri KCTC5064.

Article Snippet: The study investigated how LAB fermented WF ( ) affects the growth inhibition of various pathogens, including E. coli O157:H7 (ATCC 43895), L. monocytogenes (ATCC 7644), S. typhimurium (ATCC 35987), and V. parahaemolyticus (ATCC 35118), using the broth dilution technique.

Techniques:

Journal: Frontiers in Microbiology

Article Title: Woodfordia fruticosa fermented with lactic acid bacteria impact on foodborne pathogens adhesion and cytokine production in HT-29 cells

doi: 10.3389/fmicb.2024.1346909

Figure Lengend Snippet: Foodborne pathogen adhesion inhibition on HT-29 cells by LAB-fermented WF. (A) Adhesion efficiency in HT-29 cells after exposure to Escherichia coli O157:H7 (ATCC 43895) (CT) alone and in combination with LAB-fermented WF. (B) Adhesion efficiency in HT-29 cells after exposure to Listeria monocytogenes (ATCC 7644) (CT) alone and in combination with LAB-fermented WF. (C) Adhesion efficiency in HT-29 cells after exposure to Salmonella typhimurium (ATCC 35987) (CT) alone and in combination with LAB-fermented WF. (D) Adhesion efficiency in HT-29 cells after exposure to Vibrio parahaemolyticus (ATCC 35118) (CT) alone and in combination with LAB-fermented WF. Values are expressed as mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001. WFLR, Woodfordia fruticosa flower fermented with Lacticaseibacillus rhamnosus ATCC9595; WFLA, Woodfordia fruticosa flower fermented with Lactobacillus acidophilus ATCC 4356; WFLC, Woodfordia fruticosa flower fermented with Lactobacillus casei ATCC 7469; WFLB, Woodfordia fruticosa flower fermented with Lactobacillus buchneri KCTC5064.

Article Snippet: The study investigated how LAB fermented WF ( ) affects the growth inhibition of various pathogens, including E. coli O157:H7 (ATCC 43895), L. monocytogenes (ATCC 7644), S. typhimurium (ATCC 35987), and V. parahaemolyticus (ATCC 35118), using the broth dilution technique.

Techniques: Inhibition

Journal: Frontiers in Microbiology

Article Title: Woodfordia fruticosa fermented with lactic acid bacteria impact on foodborne pathogens adhesion and cytokine production in HT-29 cells

doi: 10.3389/fmicb.2024.1346909

Figure Lengend Snippet: IL-6 gene expression in HT-29 cells exposed to LAB-fermented WF and foodborne pathogens. (A) IL-6 gene expression of LAB-fermented WF to HT-29 cells after exposure to Escherichia coli O157:H7 (ATCC 43895) (CT) alone and in combination with LAB-fermented WF. (B) IL-6 gene expression of LAB-fermented WF to HT-29 cells after exposure to Listeria monocytogenes (ATCC 7644) (CT) alone and in combination with LAB-fermented WF. (C) IL-6 gene expression of LAB-fermented WF to HT-29 cells after exposure to Salmonella typhimurium (ATCC 35987) (CT) alone and in combination with LAB-fermented WF. (D) IL-6 gene expression of LAB-fermented WF to HT-29 cells after exposure to Vibrio parahaemolyticus (ATCC 35118) (CT) alone and in combination with LAB-fermented WF. Values are expressed as mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001. WFLR, Woodfordia fruticosa flower fermented with Lacticaseibacillus rhamnosus ATCC9595; WFLA, Woodfordia fruticosa flower fermented with Lactobacillus acidophilus ATCC 4356; WFLC, Woodfordia fruticosa flower fermented with Lactobacillus casei ATCC 7469; WFLB, Woodfordia fruticosa flower fermented with Lactobacillus buchneri KCTC5064.

Article Snippet: The study investigated how LAB fermented WF ( ) affects the growth inhibition of various pathogens, including E. coli O157:H7 (ATCC 43895), L. monocytogenes (ATCC 7644), S. typhimurium (ATCC 35987), and V. parahaemolyticus (ATCC 35118), using the broth dilution technique.

Techniques: Gene Expression

Journal: Frontiers in Microbiology

Article Title: Woodfordia fruticosa fermented with lactic acid bacteria impact on foodborne pathogens adhesion and cytokine production in HT-29 cells

doi: 10.3389/fmicb.2024.1346909

Figure Lengend Snippet: Antibacterial activity of WF extracts at various ethanol concentrations (A) . Inhibitory effects of LAB-fermented WF on foodborne pathogens [ Escherichia coli O157:H7 (ATCC 43895) (B) , Listeria monocytogenes (ATCC 7644) (C) , Salmonella typhimurium (ATCC 35987) (D) , and Vibrio parahaemolyticus (ATCC 35118) (E) ]. Values are expressed as mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001. ET, 50% ethanol solution; WF, Woodfordia fruticosa ; WFLR, Woodfordia fruticosa flower fermented with Lacticaseibacillus rhamnosus ATCC9595; WFLA, Woodfordia fruticosa flower fermented with Lacticaseibacillus acidophilus ATCC 4356; WFLC, Woodfordia fruticosa flower fermented with Lacticaseibacillus casei ATCC 7469; WFLB, Woodfordia fruticosa flower fermented with Lactobacillus buchneri KCTC5064.

Article Snippet: The cultures of foodborne pathogens including Escherichia coli O157:H7 ( E. coli ; ATCC 43895), Listeria monocytogenes ( L. monocytogenes ; ATCC 7644), Salmonella Typhimurium ( S. typhimurium ; ATCC 35987), and Vibrio parahaemolyticus ( V. parahaemolyticus ; ATCC 35118) were preserved at −20°C in Tryptic soy broth (TSB; Merck, Germany), enriched with 25% (v/v) sterile glycerol (Merck, Germany).

Techniques: Activity Assay

Journal: Frontiers in Microbiology

Article Title: Woodfordia fruticosa fermented with lactic acid bacteria impact on foodborne pathogens adhesion and cytokine production in HT-29 cells

doi: 10.3389/fmicb.2024.1346909

Figure Lengend Snippet: Impact of LAB-fermented WF on HT-29 cell viability with and without foodborne pathogens at 24 and 48 h. (A) HT-29 cell viability in the absence of foodborne pathogens. CT refers to only HT-29 cells. (B) HT-29 cell viability after exposure to Escherichia coli O157:H7 (ATCC 43895) (CT) alone and in combination with LAB-fermented WF. (C) HT-29 cell viability after exposure to Listeria monocytogenes (ATCC 7644) (CT) alone and in combination with LAB-fermented WF. (D) HT-29 cell viability after exposure to Salmonella typhimurium (ATCC 35987) (CT) alone and in combination with LAB-fermented WF. (E) HT-29 cell viability after exposure to Vibrio parahaemolyticus (ATCC 35118) (CT) alone and in combination with LAB-fermented WF. Values are expressed as mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001. WFLR, Woodfordia fruticosa flower fermented with Lacticaseibacillus rhamnosus ATCC9595; WFLA, Woodfordia fruticosa flower fermented with Lactobacillus acidophilus ATCC 4356; WFLC, Woodfordia fruticosa flower fermented with Lactobacillus casei ATCC 7469; WFLB, Woodfordia fruticosa flower fermented with Lactobacillus buchneri KCTC5064.

Article Snippet: The cultures of foodborne pathogens including Escherichia coli O157:H7 ( E. coli ; ATCC 43895), Listeria monocytogenes ( L. monocytogenes ; ATCC 7644), Salmonella Typhimurium ( S. typhimurium ; ATCC 35987), and Vibrio parahaemolyticus ( V. parahaemolyticus ; ATCC 35118) were preserved at −20°C in Tryptic soy broth (TSB; Merck, Germany), enriched with 25% (v/v) sterile glycerol (Merck, Germany).

Techniques:

Journal: Frontiers in Microbiology

Article Title: Woodfordia fruticosa fermented with lactic acid bacteria impact on foodborne pathogens adhesion and cytokine production in HT-29 cells

doi: 10.3389/fmicb.2024.1346909

Figure Lengend Snippet: Foodborne pathogen adhesion inhibition on HT-29 cells by LAB-fermented WF. (A) Adhesion efficiency in HT-29 cells after exposure to Escherichia coli O157:H7 (ATCC 43895) (CT) alone and in combination with LAB-fermented WF. (B) Adhesion efficiency in HT-29 cells after exposure to Listeria monocytogenes (ATCC 7644) (CT) alone and in combination with LAB-fermented WF. (C) Adhesion efficiency in HT-29 cells after exposure to Salmonella typhimurium (ATCC 35987) (CT) alone and in combination with LAB-fermented WF. (D) Adhesion efficiency in HT-29 cells after exposure to Vibrio parahaemolyticus (ATCC 35118) (CT) alone and in combination with LAB-fermented WF. Values are expressed as mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001. WFLR, Woodfordia fruticosa flower fermented with Lacticaseibacillus rhamnosus ATCC9595; WFLA, Woodfordia fruticosa flower fermented with Lactobacillus acidophilus ATCC 4356; WFLC, Woodfordia fruticosa flower fermented with Lactobacillus casei ATCC 7469; WFLB, Woodfordia fruticosa flower fermented with Lactobacillus buchneri KCTC5064.

Article Snippet: The cultures of foodborne pathogens including Escherichia coli O157:H7 ( E. coli ; ATCC 43895), Listeria monocytogenes ( L. monocytogenes ; ATCC 7644), Salmonella Typhimurium ( S. typhimurium ; ATCC 35987), and Vibrio parahaemolyticus ( V. parahaemolyticus ; ATCC 35118) were preserved at −20°C in Tryptic soy broth (TSB; Merck, Germany), enriched with 25% (v/v) sterile glycerol (Merck, Germany).

Techniques: Inhibition

Journal: Frontiers in Microbiology

Article Title: Woodfordia fruticosa fermented with lactic acid bacteria impact on foodborne pathogens adhesion and cytokine production in HT-29 cells

doi: 10.3389/fmicb.2024.1346909

Figure Lengend Snippet: IL-6 gene expression in HT-29 cells exposed to LAB-fermented WF and foodborne pathogens. (A) IL-6 gene expression of LAB-fermented WF to HT-29 cells after exposure to Escherichia coli O157:H7 (ATCC 43895) (CT) alone and in combination with LAB-fermented WF. (B) IL-6 gene expression of LAB-fermented WF to HT-29 cells after exposure to Listeria monocytogenes (ATCC 7644) (CT) alone and in combination with LAB-fermented WF. (C) IL-6 gene expression of LAB-fermented WF to HT-29 cells after exposure to Salmonella typhimurium (ATCC 35987) (CT) alone and in combination with LAB-fermented WF. (D) IL-6 gene expression of LAB-fermented WF to HT-29 cells after exposure to Vibrio parahaemolyticus (ATCC 35118) (CT) alone and in combination with LAB-fermented WF. Values are expressed as mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001. WFLR, Woodfordia fruticosa flower fermented with Lacticaseibacillus rhamnosus ATCC9595; WFLA, Woodfordia fruticosa flower fermented with Lactobacillus acidophilus ATCC 4356; WFLC, Woodfordia fruticosa flower fermented with Lactobacillus casei ATCC 7469; WFLB, Woodfordia fruticosa flower fermented with Lactobacillus buchneri KCTC5064.

Article Snippet: The cultures of foodborne pathogens including Escherichia coli O157:H7 ( E. coli ; ATCC 43895), Listeria monocytogenes ( L. monocytogenes ; ATCC 7644), Salmonella Typhimurium ( S. typhimurium ; ATCC 35987), and Vibrio parahaemolyticus ( V. parahaemolyticus ; ATCC 35118) were preserved at −20°C in Tryptic soy broth (TSB; Merck, Germany), enriched with 25% (v/v) sterile glycerol (Merck, Germany).

Techniques: Gene Expression

Journal: PeerJ

Article Title: MiR-525-5p inhibits diffuse large B cell lymphoma progression via the Myd88/NF-κB signaling pathway

doi: 10.7717/peerj.16388

Figure Lengend Snippet: (A) The wild or mutant sequences of Myd88. (B) Luciferase reporter gene analysis validate the relationship of miR-525-5p and Myd88. (C and D) Myd88 mRNA and protein expression was detected by RT-qPCR and Western blotting. (E) Myd88 protein expression. (F) The mRNA expression of Myd88 in lymphoid cancer cell lines (OCl-LY7, FARAGE and U2932) and human lymphoblastoid B cells (GM12878). N = 5. * P < 0.01.

Article Snippet: Myd88 siRNA (F: CCG GGC CTA TCG CTG TTC TTG AAT TCA AGA GAT TCA AGA ACA GCG ATA GGC TTT TTT GGT ACC; R: AAT TGG TAC CAA AAA AGC CTA TCG CTG TTC TTG AAT CTC TTG AAT TCA AGA ACA GCG ATA GGC) and scramble were purchased from Santa Cruz Biotechnology, Inc. A total of 20 pairs of tumor tissues and paracancerous tissues were obtained from patients.

Techniques: Mutagenesis, Luciferase, Expressing, Quantitative RT-PCR, Western Blot

Journal: PeerJ

Article Title: MiR-525-5p inhibits diffuse large B cell lymphoma progression via the Myd88/NF-κB signaling pathway

doi: 10.7717/peerj.16388

Figure Lengend Snippet: The U2932 cells were transfected with Myd88 overexpression vector (OE-Myd88) or Myd88 siRNA (si-Myd88) for 24 h, respectively. (A) Myd88 protein expression was detected by Western blotting. (B) CCK-8 assay was used to detect cell proliferation. (C) Cell invasion was detected by Transwell invasion assay. (D) Cell apoptosis was detected by flow cytometry. (E) Cell clonogenic capacity was detected by cell colony formation assay. N = 5. * P < 0.01.

Article Snippet: Myd88 siRNA (F: CCG GGC CTA TCG CTG TTC TTG AAT TCA AGA GAT TCA AGA ACA GCG ATA GGC TTT TTT GGT ACC; R: AAT TGG TAC CAA AAA AGC CTA TCG CTG TTC TTG AAT CTC TTG AAT TCA AGA ACA GCG ATA GGC) and scramble were purchased from Santa Cruz Biotechnology, Inc. A total of 20 pairs of tumor tissues and paracancerous tissues were obtained from patients.

Techniques: Transfection, Over Expression, Plasmid Preparation, Expressing, Western Blot, CCK-8 Assay, Transwell Invasion Assay, Flow Cytometry, Colony Assay

Journal: PeerJ

Article Title: MiR-525-5p inhibits diffuse large B cell lymphoma progression via the Myd88/NF-κB signaling pathway

doi: 10.7717/peerj.16388

Figure Lengend Snippet: The U2932 cells were transfected with miR-525-5p mimic alone or together with Myd88 overexpression vector. (A) The protein expression of Myd88 and NF-κB. (B) CCK-8 assay was used to detect cell proliferation. (C) Cell invasion was detected by Transwell invasion assay. (D) Cell apoptosis was detected by flow cytometry. N = 5. * P < 0.01.

Article Snippet: Myd88 siRNA (F: CCG GGC CTA TCG CTG TTC TTG AAT TCA AGA GAT TCA AGA ACA GCG ATA GGC TTT TTT GGT ACC; R: AAT TGG TAC CAA AAA AGC CTA TCG CTG TTC TTG AAT CTC TTG AAT TCA AGA ACA GCG ATA GGC) and scramble were purchased from Santa Cruz Biotechnology, Inc. A total of 20 pairs of tumor tissues and paracancerous tissues were obtained from patients.

Techniques: Transfection, Over Expression, Plasmid Preparation, Expressing, CCK-8 Assay, Transwell Invasion Assay, Flow Cytometry

Journal: PeerJ

Article Title: MiR-525-5p inhibits diffuse large B cell lymphoma progression via the Myd88/NF-κB signaling pathway

doi: 10.7717/peerj.16388

Figure Lengend Snippet: A total of 10 adult nude mice (4–6 weeks; 18–22 g; Wuhan Experimental Animal Center; Wuhan, China) were randomly divided into two groups ( N = 5 per group) including NC mimic group and miR-525-5p mimic group. A total of 10 adult nude mice were randomly divided into two groups ( N = 5 per group) including NC mimic group and miR-525-5p mimic group. (A) Tumor images on the 35th day after injection. (B) Tumor volume. (C) Tumor weight. (D) MiR-525-5p expression. (E) Myd88 and NF-κB protein expression. N = 5. * P < 0.01.

Article Snippet: Myd88 siRNA (F: CCG GGC CTA TCG CTG TTC TTG AAT TCA AGA GAT TCA AGA ACA GCG ATA GGC TTT TTT GGT ACC; R: AAT TGG TAC CAA AAA AGC CTA TCG CTG TTC TTG AAT CTC TTG AAT TCA AGA ACA GCG ATA GGC) and scramble were purchased from Santa Cruz Biotechnology, Inc. A total of 20 pairs of tumor tissues and paracancerous tissues were obtained from patients.

Techniques: Injection, Expressing

Journal: Arteriosclerosis, Thrombosis, and Vascular Biology

Article Title: Sleep Deprivation Promotes Endothelial Inflammation and Atherogenesis by Reducing Exosomal miR-182-5p

doi: 10.1161/atvbaha.123.319026

Figure Lengend Snippet: Figure 4. MYD88 (myeloid differentiation factor 88) was the target gene of miR-182-5p in endothelial cells. A, RNA sequencing was performed in human umbilical vein endothelial cells (HUVECs) treated with ox-LDL (oxidized low-density lipoprotein) for 12 hours, followed by SC-exo or SD-exo treatment for 12 hours (n=4, biological replicates). B, Screening scheme for putative target genes that might contribute to the anti-inflammatory effects of miR-182-5p. C, Representative western blots, performed by the Wes automatic protein expression analysis system, and quantified data showing protein expressions of MYD88, the putative miR-182-5p target gene, and its downstream 2 genes in HUVECs treated with miR-182-5p mimic, mimic negative control (NC), miR-182-5p inhibitor, or inhibitor NC (n=6, biological replicates). D, Luciferase assays of 293T cells co-transfected with miR-182-5p mimic or mimic NC and reporter plasmids containing 3’UTR wild type or mutated miR-182-5p-binding sites for MYD88, the putative target gene (n=6, biological replicates). The binding sites of WT and mutants were shown in the square. E, The release of IL (interleukin-1)-18 and IL-1β induced by ox-LDL in HUVECs transfected with siRNA of MYD88 (si-MYD88) or siRNA negative control (si-NC; n=5, biological replicates). Data are expressed as means±SD. One-way ANOVA followed by a post-hoc test was used to determine the significance among different groups of C. Two-way ANOVA followed by a post-hoc test was used for statistical analysis of D. Kruskal-Wallis test was used for statistical analysis of E.

Article Snippet: After 48 hours, cells were harvested and firefly and renilla luciferase activities were analyzed with the Dual-Luciferase Reporter Assay System (Promega) by using Synergy H1 microplate reader (BioTek), and the quantitative comparison of these 2 luciferase activities provided results reflecting the inhibition of miRNA on the target gene, which were normalized by the Renilla/Firefly luciferase signal. siRNA Knockdown of myD88 For loss-of-function study, HUVECs were transfected with myD88-specific siRNA (Santa Cruz, sc-35986) or scrambled control oligonucleotides using Lipofectamine 3000 according to the manufacturer’s instructions.

Techniques: RNA Sequencing, Western Blot, Expressing, Negative Control, Luciferase, Transfection, Binding Assay